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The entrance to the ants' final nest was along the concrete spillways of the Los Angeles River between the First and Seventh Street Bridges, east of downtown. The depiction of the Chihuahua Desert of southern New Mexico is actually the Mojave Desert near Palmdale, California.

Mercy Hospital was a real institution and is now Brownsville Medical Center. The geography of South Texas is unusually accurate for Hollywood. Whitmore wore "lifts" on his shoes to compensate for the height difference between himself and Arness.

It has also been noted that Whitmore employed bits of "business" (hand gestures and motions) during scenes in which he appeared to draw attention to his character when not speaking.

The Wilhelm scream, created three years earlier for the film Distant Drums, is used multiple times during the action sequences, e.g., when a wooden beam falls on top of a soldier in the storm-drain sequence.

The giant ants were constructed and operated by unseen technicians supervised by Ralph Ayers, and were actually purpleish-green in color.

However, during the climactic battle sequence in the Los Angeles sewers, there is a brief shot of one ant moving in the foreground with its side removed, revealing its mechanical interior.

Excerpts and References:
wikipedia.org, imdb.com

JOHNNY (on radio): Car 5-W, code one.

BLACKBURN: Car 5-W to 3018, go ahead Johnny.

JOHNNY (on radio): There's a trailer about three miles ahead of you pulled off to the side of the road. I didn't see anyone around it. You better check it out. Ten-ten.

BLACKBURN: Okay Johnny. Ten-ten.

BLACKBURN: What's the matter with her?

PETERSON: I don't know.

BLACKBURN: Sunstroke?

PETERSON: No, she's not sunburned. She couldn't have been out in the sun very long. It looks like she's in shock.

BLACKBURN: Yea. Say, Johnny spotted a car and trailer up ahead, maybe she's from there.

PETERSON: Yea, maybe so.

They arrive at the trailer.

BLACKBURN: No use waking her up unless somebody can identify her. I'll check it.

Black burn motions to Peterson to come look.

PETERSON: What is it?

BLACKBURN: Have a look.